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Neutrophil-to-lymphocyte ratio (NLR) is an accessible and cost-effective biomarker.
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High NLR on admission is associated with severe COVID-19 and mortality.
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NLR is recommended for use in resource-limited healthcare settings.
Abstract
Background
The neutrophil-to-lymphocyte ratio (NLR), an inflammatory marker, was suggested to be predictive of severity and mortality in COVID-19 patients. Here, we investigated whether NLR levels on admission could predict the severity and mortality of COVID-19 patients.
Methods
A literature search was conducted on 23 July 2020 to retrieve all published articles, including grey literature and preprints, investigating the association between on-admission NLR values and severity or mortality in COVID-19 patients. A meta-analysis was performed to determine the overall standardized mean difference (SMD) in NLR values and the pooled risk ratio (RR) for severity and mortality with the 95% Confidence Interval (95%CI).
Results
A total of 38 articles, including 5699 patients with severity outcomes and 6033 patients with mortality outcomes, were included. The meta-analysis showed that severe and non-survivors of COVID-19 had higher on-admission NLR levels than non-severe and survivors (SMD 0.88; 95%CI 0.72–1.04; I2 = 75.52% and 1.87; 95%CI 1.25–2.49; I2 = 97.81%, respectively). Regardless of the different NLR cut-off values, the pooled mortality RR in patients with elevated vs. normal NLR levels was 2.74 (95%CI 0.98–7.66).
Conclusion
High NLR levels on admission were associated with severe COVID-19 and mortality. Further studies need to focus on determining the optimal cut-off value for NLR before clinical use.
]. COVID-19 is caused by the Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2), a novel virus in the same cluster as the SARS-CoV-1 and MERS-CoV, that previously caused outbreaks in 2003 and 2012 [
]. The clinical manifestations of COVID-19 patients range from asymptomatic to severe symptoms. A minority (30%) progresses into severe manifestations such as acute respiratory distress syndrome (ARDS), severe pneumonia, septic shock, coagulopathy, and death [
]. Early risk stratification for COVID-19 patients upon hospital admission is the key to providing optimal interventions and to carefully allocate the ongoing scarce human and technical resources [
]. This would ensure that the limited available resources are given to the right patients. The neutrophil-to-lymphocyte ratio (NLR) is an inflammatory marker derived from combining the absolute blood neutrophil and lymphocyte counts, two routinely performed parameters in clinical settings. Recently, studies have reported that NLR levels were higher in more severe patients and were suggested to confer a prognostic value in COVID-19 patients [
Neutrophil-to-lymphocyte ratio and lymphocyte-to-C-reactive protein ratio in patients with severe coronavirus disease 2019 (COVID-19): A meta-analysis.
]. The underlying pathophysiology that justifies for the clinical use of this biomarker is that severe COVID-19 patients were more likely to present with higher levels of inflammation upon hospital admission. Therefore, obtaining NLR levels on hospital admission could allow early risk stratification, identifying patients who should be prioritized for scarce resources.
We performed a systematic review and meta-analysis to investigate whether clinical outcomes of severity and mortality in COVID-19 patients can be predicted with on-admission NLR values.
2. Methods
This systematic review and meta-analysis is reported according to the Preferred Reporting Items for Systematic Review and Meta-analysis (PRISMA) Checklist (Table S1). Before writing this review, a detailed protocol was created and registered on the International Prospective Register of Systematic Reviews (PROSPERO) on 1 June 2020 (CRD42020189218).
2.1 Eligibility criteria
We included all research papers investigating adult patients (older than 18 years old) with COVID-19 (diagnosed with RT-PCR) that contain information on the NLR value at the time of hospital admission and clinical grouping of outcomes with a clinically validated definition of COVID-19 severity or death. The following articles were excluded from this review: non-research letters, correspondences, case reports, review articles, and original articles with samples below 20. Due to the limited resources, we only included articles published in English.
2.2 Search strategy
We searched for all published articles, including preprints and grey literature, from electronic databases on 23 July 2020. Peer-reviewed papers were sought from four databases (Ovid MEDLINE, Ovid EMBASE, SCOPUS, and the Cochrane Library); preprints were searched from three databases (MedRxiv, BioRxiv, and SSRN); and grey literature was searched from two databases (WHO COVID-19 Global Research Database, and the Centers for Disease Control and Prevention COVID-19 Research Article Database). The search strategies used were developed from the following key concepts: “COVID-19”, “Neutrophil-to-lymphocyte ratio”, “Severity”, and “Mortality” (Table S2). Manual hand-searching and forward and backward tracing of citations from relevant articles were also done to identify additional studies.
2.3 Study selection
All articles retrieved from the systematic searches of electronic databases were exported to EndNote X9 bibliographic and reference manager. Following deduplication, the titles and abstracts were screened independently by three reviewers (DMS, ADW, NAAI), and the remaining articles were screened for its full text against the eligibility criteria. Any disagreements were resolved through discussion until a common consensus was reached.
2.4 Quality assessment
The studies were critically appraised using the Newcastle Ottawa Scale (NOS) by three independent reviewers (DMS, ADW, NAAI), and when there was a discrepancy in the assessment score, discussions were done to reach an agreement.
2.5 Data extraction and synthesis
Prior to the data extraction, a customized, standardized data extraction form was developed. The data extracted included: first author, year of study, publication type, study location, study design, baseline population characteristics (including age, gender, and underlying diseases such as diabetes mellitus, hypertension, and cardiovascular diseases), exposures, and outcomes. The exposure was defined as the NLR value on admission to the hospital, presented as either continuous or dichotomized NLR values. The outcomes of interest were severe COVID-19 and mortality. Severe COVID-19 was defined as patients who met any of the following features: (1) respiratory rate > 30 breaths per minute; (2) oxygen saturation < 93% (ambient air); (3) ratio of the partial pressure of arterial blood oxygen (PaO2)/oxygen concentration (FiO2) ≤300 mmHg [
]. Due to different severity grouping criteria among studies, non-severe COVID-19 included patients with either mild, moderate, common, ordinary, or any combination. Meanwhile, severe COVID-19 included patients in severe, critical, or a combination of the two. Additionally, for studies that performed and reported receiver operating characteristic (ROC) analysis on either severity or mortality, we extracted the optimal NLR cut-off values, the area under the curve (AUC), sensitivity, and specificity.
2.6 Statistical analysis
The quantitative data were exported to Stata version 16, and a meta-analysis of studies was performed. For non-normal data, we extrapolated the mean and standard deviation from the available median and interquartile range (IQR) using the method by Hozo et al. [
]. For studies that reported the means of NLR among groups, pooled standardized mean difference (SMD) and the 95% Confidence Interval (95%CI) were obtained using the inverse variance method. For NLR values reported as dichotomized variables, the pooled risk ratio (RR) with the 95%CI was obtained using the Mantel-Haenszel method. Statistical heterogeneity was determined using the Cochrane chi-square and I2 with cut-off values for I2 of greater than 50% to be considered significantly heterogeneous. In this study, we used the DerSimonian-Laird random-effects model if I2 was greater than 50%, and the fixed-effects model if I2 was less than or equal to 50%. Sensitivity analysis was done by omitting one study at a time to identify any source of heterogeneity and restricting the studies to only peer-reviewed papers and only studies with low risk of bias. Publication bias was assessed qualitatively using the funnel plot by comparing the SMD and the standard error of the natural log of SMD [SE(SMD)], and quantitatively using Egger's linear regression test to evaluate the presence of small-study effects. A random-effects meta-regression was performed for the following potential confounders: age, gender, diabetes mellitus, hypertension, and cardiovascular disease. A statistically significant difference was considered if a two-tailed p < 0.05.
3. Results
3.1 Search selection
A total of 203 papers were identified from the peer-reviewed databases, and an additional nine papers were retrieved from manual hand-searching, preprint, and grey literature databases. After removing duplicates, 102 unique articles were reviewed for its titles and abstracts, leaving a total of 55 articles eligible for full-text review. After a thorough evaluation, according to the eligibility criteria, 38 papers met the inclusion criteria (Fig. 1).
Fig. 1Prisma diagram for study selection. A systematic literature search was done on 23 July 2020 to identify peer-reviewed papers, preprints, and grey literature.
This systematic review included 38 articles incorporating 5699 patients with severity outcomes and 6033 patients with mortality outcomes. Thirty-two articles were peer-reviewed [
Development and validation of a risk factor-based system to predict short-term survival in adult hospitalized patients with COVID-19: a multicenter, retrospective, cohort study.
Predictive value of the neutrophil-to-lymphocyte ratio (NLR) for diagnosis and worse clinical course of the COVID-19: findings from ten provinces in China.
The characteristics and death risk factors of 132 COVID-19 pneumonia patients with comorbidities: a retrospective single center analysis in Wuhan, China.
]; 23 articles compared NLR values on admission in severe vs. non-severe COVID-19 patients, 13 articles compared NLR values on admission in survivors vs. non-survivors of COVID-19, and only two articles compared the NLR values on admission in regard to both the severity and mortality of COVID-19 patients [
The characteristics and death risk factors of 132 COVID-19 pneumonia patients with comorbidities: a retrospective single center analysis in Wuhan, China.
] (Table 1, Table 2, Table S3–4). Studies with severity as the outcome measure had a median risk of bias score of 7 (IQR 5.5–8.5; range 5–9). On the other hand, studies comparing the NLR value on admission in survivors and non-survivors of COVID-19 had a median risk of bias score of 8 (IQR 7–9; range 6–9) (Table S5).
Table 1Baseline characteristics of included studies comparing severe and non-severe COVID-19 patients
First Author
Study Location
Groups
Sample (N)
Male %
Age (years) Mean ± SD / Median (IQR)
DM N (%)
HT N (%)
CVD N (%)
NLR Value Mean ± SD / Median (IQR)
NOS Score
Qin C et al
China
Severe
286
54
61 (51–69)
53 (19)
105 (37)
24 (8)
5.5 (3.3–10.0)
5
Non-severe
166
48
53 (41–62)
22 (13)
30 (18)
3 (2)
3.2 (1.8–4.9)
Zhang Y et al
China
Severe
31
65
65 ± 13
NR
NR
NR
7.58 ± 7.04
7
Mild
84
35
44 ± 15
NR
NR
NR
2.28 ± 1.29
Yang AP et al
China
Severe
24
75
58 ± 12
13 (54)
16 (67)
9 (38)
20.7 ± 24.1
7
Non-severe
69
55
42 ± 19
8 (12)
7 (10)
4 (6)
4.8 ± 3.5
Gong J et al
China
Severe
28
57
64 (55–72)
NR
NR
NR
3.7 (2.0–6.7)
7
Non-severe
161
45
45 (33–62)
NR
NR
NR
1.9 (1.4–2.9)
Zhu Z et al
China
Severe
16
56
58 ± 12
0 (0)
8 (50)
2 (13)
4.24 (3.00–10.87)
7
Non-severe
111
66
50 ± 16
10 (9)
23 (21)
4 (4)
2.75 (1.90–3.95)
Sun S et al
China
Severe
27
67
62 (53–71)
NR
NR
NR
8.71 (3.77–14.4)
5
Common
89
47
47 (37–55)
NR
NR
NR
2.41 (1.73–3.47)
Liu F et al
China
Severe
19
79
63 (40–66)
3 (16)
6 (32)
1 (5)
3.4 (2.8–5.8)
8
Non-severe
115
42
50 (36–64)
7 (6)
21 (18)
4 (4)
2.7 (1.8–3.7)
Fu J et al
China
Severe
16
63
52 ± 13
4 (5)
7 (9)
NR
6.29 ± 3.72
6
Mild/moderate
59
59
45 ± 14
2.33 ± 1.22
Ding X et al
China
Severe
15
60
67 (55–76)
5 (7)
9 (13)
6 (8)
3.6 (2.4–9.6)
7
Non-severe
57
42
46 (35–60)
1.9 (1.3–2.9)
Chen R et al
China
Critical
48
79
61 ± 14
5 (10)
23 (48)
7 (15)
16.06 (11.26–26.35)
9
Severe
155
60
61 ± 14
23 (15)
52 (34)
14 (9)
8.96 (4.62–17.04)
Mild/moderate
345
53
67 ± 12
33 (10)
73 (21)
14 (4)
3.37 (2.05–6.65)
Shang W et al
China
Severe
139
59
64 (54–73)
20 (14)
45 (32)
25 (18)
4.75 (2.51–9.42)
7
Non-severe
304
45
58 (47–67)
43 (14)
86 (28)
19 (6)
2.38 (1.57–3.72)
Xie G et al
China
Severe
12
83
52 (35–66)
2 (17)
4 (33)
2 (17)
3.0 (1.56–6.55)
5
Non-severe
85
51
45 (32–60)
3 (4)
16 (19)
5 (6)
2.74 (2.03–3.96)
Xie L et al
China
Severe
51
57
NR
8 (16)
12 (24)
6 (12)
7.90 ± 10.20
5
Non-severe
322
52
NR
21 (7)
59 (18)
12 (4)
2.93 ± 1.80
Zhou Y et al
China
Moderate
140
39
56 ± 14
NR
NR
NR
3.1 ± 2.41
5
Severe
123
47
64 ± 14
NR
NR
NR
11.66 ± 27.66
Critically severe
41
61
65 ± 13
NR
NR
NR
Wu S et al
China
Severe or critical
67
67
66 (54–73)
8 (12)
22 (33)
6 (9)
5.8 (3.3–13.0)
7
Moderate
203
42
61 (50–68)
27 (13)
59 (29)
5 (3)
2.2 (1.5–3.4)
Kong M et al
China
Severe
87
52
68 ± 12
18 (21)
47 (54)
11 (13)
6.6 (2.1–11.1)
7
Mild
123
48
53 ± 16
9 (7)
32 (26)
9 (7)
3.3 (1.0–3.4)
Wang F et al
China
Severe
70
64
60 (49–64)
NR
NR
NR
2.72 (1.87–4.37)
8
Non-severe
253
43
41 (32–56)
NR
NR
NR
1.72 (1.19–2.53)
Liao D et al
China
Critical
86
71
68 (61–78)
17 (20)
28 (33)
8 (9)
16.02 (6.49–24.79)
7
Severe
145
52
67 (58–76)
30 (21)
49 (34)
8 (6)
4.71 (2.62–7.78)
Moderate
149
46
56 (42–68)
14 (9)
37 (25)
4 (3)
2.67 (1.69–4.08)
Ok F et al
Turkey
Severe
54
44
68 ± 15
3 (13)
10 (44)
6 (26)
6.1 (5.1)
7
Non-severe
85
45
47 ± 16
4 (7)
6 (10)
2 (3)
2.46 (2.3)
Guner R et al
Turkey
SARI/Critical
50
66
62 ± 12
10 (20)
16 (32)
20 (40)
5.6 (1.5–38)#
6
Mild/pneumonia
172
58
48 ± 16
20 (12)
36 (21)
36 (21)
2.5 (0.4–28)#
Song CY et al
China
Severe
42
71
56 (48–64)
4 (10)
22 (52)
4 (10)
8.2 (3.9–19.2)
6
Non-severe
31
52
48 (37–59)
2 (7)
4 (13)
1 (3)
3.0 (1.9–5.5)
Liu J et al
China
Severe
79
58
65 (54–71)
13 (17)
37 (47)
2 (3)
8.83 (4.20–15.53)
7
Common
43
61
55 (38–66)
2 (5)
13 (30)
0 (0)
3.11 (1.96–5.00)
Ma Y et al
China
Severe
63
46
53 ± 13
6 (10)
15 (24)
NR
9.38 ± 10.52
6
Ordinary (Moderate)
486
54
45 ± 15
23 (5)
75 (15)
NR
3.58 ± 3.07
Mild
86
43
39 ± 18
6 (7)
15 (17)
NR
2.73 ± 2.28
Chen C et al
China
Critical
23
65
68 (63–79)
9 (39)
19 (83)
7 (30)
7.08 (3.48–12.89)
8
Mild
109
56
62 (53–70)
36 (27)
71 (65)
24 (22)
4.10 (2.19–7.51)
Wang J et al
China
Severe
8
49
45 (25–61)
5 (9)
13 (24)
1 (2)
2.4 (1.4–16.2)
5
Moderate
25
2.3 (1.7–2.9)
Mild
22
1.8 (0.9–2.8)
CVD = Cardiovascular Disease; DM = Diabetes Mellitus; HT = Hypertension; IQR = Interquartile Range; NLR = Neutrophil-to-lymphocyte ratio; NOS = Newcastle-Ottawa Scale; NR = Not Reported; SD = Standard Deviation; # = min and max data.
Table 2Baseline characteristics of included studies comparing survivors and non-survivors of COVID-19 patients
First Author
Study Location
Groups
Sample (N)
Male %
Age (years) Mean ± SD / Median (IQR)
DM N (%)
HT N (%)
CVD N (%)
NLR value Mean ± SD / Median (IQR)
NOS Score
Liu Y et al
China
NLR Tertile 1 (0.54–2.21)
82
29
48 ± 16
5 (6)
11 (13)
5 (6)
NR
8
NLR Tertile 2 (2.21–4.82)
81
49
53 ± 17
6 (7)
14 (17)
3 (4)
NR
NLR Tertile 3 (4.85–88.09)
82
61
61 ± 15
12 (15)
27 (33)
10 (12)
NR
Chen R et al
China
Survivor
445
55
54 ± 14
41 (9)
103 (23)
24 (5)
3.71 (2.27–7.54)
9
Non-survivor
103
67
67 ± 12
20 (19)
45 (44)
11 (11)
13.45 (9.33–23.60)
Huang J et al
China
Survivor
283
53
53 ± 17
31 (11)
63 (22)
14 (5)
3.3 ± 4.3
8
Non-survivor
16
69
69 ± 10
4 (25)
11 (69)
4 (25)
13.3 ± 14.3
Zhang N et al
China
Survivor
50
72
63 ± 11
5 (10)
18 (36)
11 (22)
8.4 ± 7.5
6
Non-survivor
10
70
71 ± 9
4 (40)
4 (40)
3 (30)
18.7 ± 16.6
Li L et al
China
Survivor
68
38
44 ± 13
6 (9)
0 (0)
0 (0)
2.3 (1.6–3.8)
7
Non-survivor
25
60
69 ± 11
5 (20)
5 (20)
4 (16)
3.8 (2.8–6.6)
Luo X et al
China
Survivor
214
46
51 (37–63)
27 (13)
37 (17)
13 (6)
2.96 (2.13–4.61)
8
Non-survivor
84
61
71 (64–80)
18 (21)
49 (58)
13 (16)
8.17 (6.15–10.90)
Yan X et al
China
Survivor
964
48
62 (50–70)
97 (11)
215 (22)
65 (7)
4.11 (2.44–8.12)
8
Non-survivor
40
68
68 (58–79)
10 (25)
20 (50)
10 (25)
49.06 (25.71–69.70)
Chen L et al
China
Survivor
1651
47
57 (43–66)
203 (12)
475 (29)
205 (12)$
3 (2–4)
9
Non-survivor
208
74
70 (63–78)
59 (28)
104 (50)
62 (30)$
11 (6–20)
Tatum D et al
USA
NLR ≤ 4.94
62
43
56.1 ± 15.2
NR
NR
NR
NR
6
NLR > 4.94
57
47
62.1 ± 14.1
NR
NR
NR
NR
Ullah W et al
USA
NLR < 10
141
47
63.6
49 (35)
93 (66)
27 (19)
NR
8
NLR > 11
26
62
61.6
10 (39)
14 (54)
2 (8)
NR
Ye W et al
China
Survivor
297
46
60 (50–67)
41 (14)
73 (25)
5 (2)
2.88 (1.79–6.74)
8
Non-survivor
52
69
69 (63–76)
16 (31)
30 (58)
11 (21)
14.96 (8.52–26.58)
Yang Q et al
China
Survivor
176
47
50 ± 15
28 (16)
47 (27)
6 (3)
2.98 (1.70–5.51)
8
Non-survivor
50
62
68 ± 16
17 (34)
37 (74)
7 (14)
6.18 (3.58–12.78)
Zhang S et al
China
Survivor
420
51
59 (48–67)
60 (14)
107 (26)
53 (13)
3.91 (2.07–6.79)
7
Non-survivor
96
75
67 (61–74)
16 (17)
31 (32)
14 (15)
10.99 (7.68–20.97)
Cheng B et al
China
Survivor
67
33
71 ± 7
11 (16)
39 (58)
11 (16)
4.1 ± 2.9
8
Non-survivor
51
61
73 ± 7
16 (31)
25 (49)
12 (24)
13.3 ± 14.9
Chen C et al
China
Survivor
119
56
NR
42 (35)
80 (68)
24 (20)
4.19 (2.30–7.39)
8
Non-survivor
13
69
NR
3 (23)
10 (77)
7 (54)
12.21 (3.66–14.98)
CVD = Cardiovascular Disease; DM = Diabetes Mellitus; HT = Hypertension; IQR = Interquartile Range; NLR = Neutrophil-to-lymphocyte ratio; NOS = Newcastle-Ottawa Scale; NR = Not Reported; SD = Standard Deviation; USA = United States of America; $ = included cerebrovascular disease.
3.3 Neutrophil-to-lymphocyte ratio (NLR) and severity of COVID-19
There was a total of 5699 patients from a total of 25 included articles comparing on-admission NLR levels in COVID-19 patients with different severity levels. Overall, 1805 patients (32%) had severe COVID-19, and seven studies reported significantly higher proportions of males in the severe COVID-19 group. Compared to the non-severe group, patients with severe COVID-19 were generally older and had more comorbidities, such as diabetes mellitus, hypertension, and cardiovascular diseases. All studies reported higher NLR values on admission in severe COVID-19 patients than non-severe COVID-19 patients (Table 1, Table S3). Four out of 25 studies (16%) performed a ROC analysis to determine the optimal cut-off value for NLR value to predict severity [
Predictive value of the neutrophil-to-lymphocyte ratio (NLR) for diagnosis and worse clinical course of the COVID-19: findings from ten provinces in China.
We performed a meta-analysis from 20 eligible articles comparing on-admission NLR levels in COVID-19 patients with different severity groups. From a total of 3859 patients, 1250 patients (32%) experienced severe COVID-19. The pooled analysis showed that severe COVID-19 patients had higher NLR on admission than non-severe patients, with an SMD of 0.88 (95%CI 0.72–1.04) (Fig. 2A ). However, the included studies were significantly heterogeneous (I2 = 75.52%). The funnel plot indicated there was an asymmetry with studies lying outside of the 95%CI, however, Egger's test showed a low risk of publication bias (p = 0.218) (Fig. 2B). Sensitivity analysis resulted in no significant changes to the outcome of the analysis (Table S6). Furthermore, restricting the analysis to only peer-reviewed studies and studies with low risks of bias showed similar pooled results (SMD 0.91; 95%CI 0.73–1.10; I2 = 79.01% and 0.87; 95%CI 0.77–0.96; I2 = 47.23%, respectively) (Fig. S1, S2). Meta-regression analysis showed that the association between NLR values on admission and severity in COVID-19 patients was not influenced by age (p = 0.236), gender (p = 0.895), cardiovascular diseases (p = 0.886), diabetes mellitus (p = 0.880), or hypertension (p = 0.222) (Fig. 3A , Fig. S3A−D).
Fig. 2Neutrophil-to-lymphocyte ratio (NLR) value on admission in severe vs. non-severe COVID-19 patients. A) Forest Plot for all included studies using the DerSimonian-Laird random-effect models showing elevated NLR values on admission in severe compared to non-severe COVID-19. B) Publication bias analysis of all included studies using the Funnel Plot indicating a potential publication bias.
Fig. 3Bubble plot for meta-regression. The association between NLR values on admission and severity of COVID-19 (A) and COVID-19 mortality (B) was not affected by age (p = 0.236; p = 0.595, respectively).
3.4 Neutrophil-to-lymphocyte ratio (NLR) and mortality in COVID-19 patients
From a total of 15 studies incorporating 6033 patients with NLR levels on admission in survivor and non-survivor of COVID-19 patients, 822 (14%) died in the hospital. Three of the studies (20%) reported the all-cause mortality of COVID-19 patients in dichotomized NLR values with varying cut-off values (Table 2). Generally, those with COVID-19 who died were mostly males, significantly older and had higher proportions of diabetes mellitus, hypertension, and cardiovascular diseases. All studies reported an elevated NLR level on admission in non-survivors compared to survivors.
Two studies performed a ROC analysis to determine the optimal cut-off value of 11.75 with an AUC value of 0.945 (95%CI 0.917–0.973), a sensitivity of 97.5%, and a specificity of 78.1% [
] (Table 3). The multivariable regression comparing patients with low (<11.75) and high (>11.75) NLR levels in Yan X et al. resulted in an adjusted odds ratio (AOR) of 44.351 (95%CI 4.627–425.088) [
A meta-analysis was done on 12 eligible studies. From a total of 5502 patients, 748 (14%) were non-survivors. The meta-analysis showed that non-survivors had higher NLR on admission than survivors, with a pooled SMD of 1.87 (95%CI 1.25–2.49). Significant heterogeneity was found among the studies (I2 = 97.81%) (Fig. 4A ). The funnel plot was visually asymmetric with studies lying outside the 95%CI, however, the calculated Egger's test showed a low risk of publication bias (p = 0.797) (Fig. 4B). Sensitivity analysis by removing one study at a time did not significantly alter the conclusion of the results (Table S7). Restricting the analysis to only peer-reviewed studies and studies with low risks of bias also showed similar pooled SMD of 1.97 (95%CI 1.27–2.66; I2 = 98.11%) and 1.93 (95%CI 1.28–2.58; I2 = 97.99%) (Fig. S4, S5). Furthermore, the association between NLR and mortality in COVID-19 was also unaffected by age (p = 0.595), gender (p = 0.644), cardiovascular diseases (p = 0.477), diabetes mellitus (p = 0.239), hypertension (p = 0.545) (Fig. 3B, Fig. S6A−D).
Fig. 4Neutrophil-to-lymphocyte ratio (NLR) value on admission in non-survivor vs. survivor of COVID-19 patients. A) Forest Plot for all included studies using the DerSimonian-Laird random-effects model showing elevated NLR values on admission in non-survivors compared to survivors of COVID-19. B) Publication bias analysis of all included studies using the Funnel Plot indicating a potential publication bias. C) Forest Plot using the DerSimonian-Laird random-effects model showing the association between NLR value on admission and all-cause mortality risk.
For the three studies with dichotomized NLR values on admission, a meta-analysis showed that patients with elevated NLR had a higher risk of mortality than those with normal NLR (RR 2.74; 95%CI 0.98–7.66), regardless of the NLR cut-off values used. Significant heterogeneity between the studies was found (I2 = 77.09%) (Fig. 4C).
4. Discussion
With an increasing number of COVID-19 cases and the limited healthcare capacity, early prediction of COVID-19 severity and mortality is crucial in the patient triage process. Scoring systems such as the Acute Physiology and Chronic Health Evaluation (APACHE) II score was suggested to be a useful clinical tool to predict in-hospital mortality in COVID-19 patients [
]. However, both scoring systems cannot be implemented in resource-limited healthcare settings, especially in the acute phase, as they heavily rely on advanced laboratory examinations such as serum electrolytes and arterial pH in the APACHE II and lactate dehydrogenase in the COVID-GRAM. Therefore, simpler tools for predicting the severity and mortality of COVID-19 patients in the early stages are urgently needed.
In this study, we performed a systematic review of 38 studies to evaluate the role of NLR levels on admission in predicting the severity and mortality of COVID-19. Our meta-analysis showed that higher NLR values on admission were associated with higher risks of severity and mortality in COVID-19 patients, suggesting that this readily available biomarker can be used to predict the prognosis of COVID-19 patients. However, the differences in NLR values on admission between the survivor and non-survivor patients were greater than those between severe and non-severe patients. Those with high NLR levels on admission had roughly two times the risk of death compared to those with normal NLR levels. Both relationships were shown to be independent of age, gender, diabetes mellitus, hypertension, and cardiovascular diseases.
COVID-19 severity is primarily affected by the innate inflammatory response of the body, where more severe cases were attributed to cytokine storm, a condition when there is an excessive immune response [
]. NLR is a known indicator of systemic inflammation that has been widely used for many conditions, such as predicting in-hospital mortality in sepsis patients, outcomes in cardiovascular diseases, and poor prognosis and higher ICU admissions in acute pancreatitis [
Neutrophil to lymphocyte ratio in relation to risk of all-cause mortality and cardiovascular events among patients undergoing angiography or cardiac revascularization: a meta-analysis of observational studies.
]. The biological mechanism underlying this association is that high NLR indicates an imbalance in the inflammatory response, which resulted from increased neutrophil and decreased lymphocyte counts. Inflammatory factors related to viral infection, such as interleukin-6, interleukin-8, and granulocyte colony-stimulating factor, could stimulate neutrophil production [
Compared with other laboratory parameters that predict the prognosis of COVID-19, such as interleukin-6, D-dimer levels, C-reactive protein, and erythrocyte sedimentation rate; NLR is more practical for clinical application as it is easily obtained in routine blood tests [
]. Due to the low cost and no need for specific assay equipment, NLR remains a simple, accessible, near real-time, and cost-effective biomarker, especially for healthcare facilities with limited medical resources [
Prognostic role of neutrophil to lymphocyte ratio and platelet to lymphocyte ratio in prostate cancer: a meta-analysis of results from multivariate analysis.
However, to date, no NLR consensus cut-off value has been established to determine normal and elevated NLR values, especially for COVID-19. In determining the optimal cut-off value of NLR, four studies used various NLR values ranging from 3.3 to 5.9 to predict severity [
Predictive value of the neutrophil-to-lymphocyte ratio (NLR) for diagnosis and worse clinical course of the COVID-19: findings from ten provinces in China.
]. This wide variation indicates that absolute NLR values measured in different populations are hardly comparable and that optimal cut-off values may vary from one population to another.
NLR values were previously reported to vary with age and sex; thus, NLR must be interpreted carefully [
Reference values of neutrophil-lymphocyte ratio, lymphocyte-monocyte ratio, platelet-lymphocyte ratio, and mean platelet volume in healthy adults in South Korea.
]. In a Chinese population, the reference range of NLR in normal males and females, from a total of 5000 people, was 0.43–2.75 and 0.37–2.87, respectively [
]. The included studies in this meta-analysis generally showed significant differences in age and gender between groups; thus, they could theoretically explain the NLR differences between groups. However, the meta-regression analysis showed that the associations between NLR and COVID-19 severity and mortality were independent of age, gender, and underlying diseases. Therefore, determining the cut-off value is essential for NLR to be used in clinical settings, allowing early risk stratification upon hospital admission.
Our meta-analysis showed significant heterogeneity among the studies. The sensitivity analysis could not determine the source of heterogeneity except for the association between on-admission NLR and severity, when restricting studies to only those with low risks of bias eliminated the heterogeneity. However, in overall, the pooled estimate results were not significantly altered even after performing the sensitivity analysis. The identification of heterogeneity among studies with mortality outcomes was not possible due to the possibility of higher variability in treatment protocols among studies with mortality outcomes compared to severity.
To date, our study is the first meta-analysis to describe the predictive values of NLR on admission for the severity and mortality of COVID-19 patients. Additionally, our results showed definitive results that can be directly applied to clinical practice. Moreover, our analysis has emphasized that the association between NLR levels on admission and poor outcomes for COVID-19 was independent of predictors, such as age, hypertension, diabetes mellitus, and cardiovascular diseases.
There are some limitations to this meta-analysis. First, we acknowledge that most of the included studies were primarily conducted in China. Thus, our data might have less clinical relevance in other countries, especially in countries with higher cases and death rates, such as in the United States of America and Europe. Second, we included preprints in the meta-analysis. However, the preprints included had low risks of bias, and further sensitivity analysis to only peer-reviewed studies showed similar results to when preprints were included. Lastly, the studies included in our meta-analysis were all retrospective, except for one, which was a prospective cohort study.
5. Conclusion
Our meta-analysis demonstrated that NLR on admission is predictive of the severity and mortality in COVID-19 patients, where higher NLR levels are associated with poor outcomes. To date, no optimal cut-off value has been validated across different populations. Therefore, prior to clinical use, further studies should be developed to obtain an exact consensus cut-off value with the optimal sensitivity and specificity. However, our findings support the use of NLR levels to perform early risk stratification in clinical settings, thus allowing patients with higher NLR to be prioritized for healthcare resource allocation.
Funding
This study was not funded by any grant.
Authors' contributions
DMS: Idea formulation, article draft writing, data collection and analysis, interpretation of the data, and critical review of the writing; JC: data collection and analysis, interpretation of the data, and critical review of the writing; ADW: article draft writing, data collection and analysis, and critical review of the writing; NAAI: article draft writing, data collection and analysis, and critical review of the writing. All authors have substantially contributed to the formulation of this manuscript. We declare no conflicts of interest.
Credit authorship contribution statement
Daniel Martin Simadibrata: Conceptualization, Data curation, Formal analysis, Writing - original draft, Writing - review & editing, Supervision. Julius Calvin: Data curation, Formal analysis, Writing - review & editing. Alya Darin Wijaya: Data curation, Writing - original draft, Writing - review & editing. Naufal Arkan Abiyyu Ibrahim: Data curation, Writing - original draft, Writing - review & editing.
Declaration of Competing Interest
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Neutrophil-to-lymphocyte ratio and lymphocyte-to-C-reactive protein ratio in patients with severe coronavirus disease 2019 (COVID-19): A meta-analysis.
Development and validation of a risk factor-based system to predict short-term survival in adult hospitalized patients with COVID-19: a multicenter, retrospective, cohort study.
Predictive value of the neutrophil-to-lymphocyte ratio (NLR) for diagnosis and worse clinical course of the COVID-19: findings from ten provinces in China.
The characteristics and death risk factors of 132 COVID-19 pneumonia patients with comorbidities: a retrospective single center analysis in Wuhan, China.
Neutrophil to lymphocyte ratio in relation to risk of all-cause mortality and cardiovascular events among patients undergoing angiography or cardiac revascularization: a meta-analysis of observational studies.
Prognostic role of neutrophil to lymphocyte ratio and platelet to lymphocyte ratio in prostate cancer: a meta-analysis of results from multivariate analysis.
Reference values of neutrophil-lymphocyte ratio, lymphocyte-monocyte ratio, platelet-lymphocyte ratio, and mean platelet volume in healthy adults in South Korea.
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